A Spectrophotometric Microdetermination of Keto Acids with 3-Methyl-2-benzothiazolone Hydrazone
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چکیده
منابع مشابه
Spectrophotometric determination of pyridoxal and pyridoxal 5'-phosphate with 3-methyl-2-benzothiazolone hydrazone hydrochloride, and their selective assay.
A spectrophotometric method with 3-methyl-2-benzothiazolone hydrazone hydrochloride was developed for the determination of pyridoxal and pyridoxal 5'-phosphate, and for the selective determination of each in the presence of the other. Pyridoxal and pyridoxal 5'-phosphate react with the reagent to yield the azine derivatives, which give characteristic absorption spectra. The highest extinction v...
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for the determination of serum or plasma triglycerides. The samples are extracted with 2propanol containing activated alumina. An AutoAnalyzer Sampler II and Proportioning Pump II are used in combination with a Gilford Model 300 N spectrophotometer and Model 3091-A debubbler flow cell. Following on-line saponification, glycerol is oxidatively cleaved with sodium metaperiodate. The resulting for...
متن کاملShort Note ( E ) - 3 - Methyl - 6 - ( 3 - oxo - 3 - ( thiophen - 2 - yl ) - 1 - propenyl ) - 2 ( 3 H ) - benzothiazolone
The title compound, (E)-3-methyl-6-(3-oxo-3-(thiophen-2-yl)-1-propenyl)-2(3H)-benzothiazolone, was synthesized by Claisen-Schmidt condensation of 3-methyl-2(3H)-benzothiazolone-6-carbaldehyde with 2-acetylthiophene in 94% yield. The structure of the target compound was confirmed using 1H-NMR, 13C-NMR, IR, MS, and elemental analysis.
متن کاملMicrodetermination of alpha-keto acids with special reference to malic, lactic, and glutamic dehydrogenases in brain.
Previous studies have indicated marked differences in the quantitative distribution of a number of enzymes, as well as other substances, in various histologically distinct areas of the nervous system (l-4). An earlier method (2) for MDH, LDH, and GDH’ in brain required 0.3, 1.5, and 10 y of brain, dry weight, respectively, for the determination of these enzymatic activities. Some of the histolo...
متن کاملSpectrophotometric Studies on the Decarboxylation of & Keto Acids
Reaction 1 is catalyzed by an enzyme widely distributed in animal tissues and present in some plants (1). Oxalacetic carboxylase, which catalyzes Reaction 2, has been found in bacteria (2), pigeon liver (3), and, to some extent, in other animal tissues as well as in plants (4). Mehler et al.’ have partially purified this enzyme from Microcowus lysocleikticus. Acetoacetic carboxylase (Reaction 3...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1967
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.31.1054